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Table 3 Immobilization of d-Hyd(co) and d-Carb via functionalized magnetic beads and hydrolysis of different substrates

From: Toward a cell-free hydantoinase process: screening for expression optimization and one-step purification as well as immobilization of hydantoinase and carbamoylase

Fraction Substrate d-Hyd(co) Substrate d-Carb
cprot (mg/mL) Aspez (mU/mg) cprot (mg/mL) Aspez (U/mg)
Crude extract PheHyd 5.00 0.36 NCPheGly 5.00 0.07
Immobilized enzyme 0.11 70.75 0.03 11.75
Crude extract BnH 5.00 0.28 NCPheAla 5.00 0.10
Immobilized enzyme 0.08 63.20 0.03 12.91
Crude extract HMH 5.00 0.76 NCSer 5.00 0.08
Immobilized enzyme 0.08 105.47 0.03 11.75