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Table 1 Purification of the d-Hyd(co) and d-Carb via Ni Sepharose beads

From: Toward a cell-free hydantoinase process: screening for expression optimization and one-step purification as well as immobilization of hydantoinase and carbamoylase

Enzyme

Fraction

A (mU/mL)

Avol (mU/mL)

Atot (mU)

cprot, tot (mg)

Aspez (mU/mg)

Recovery (%)

Purification fold

d-Hyd(co)

Crude extract

12.8

25.7

102.9

95.9

1.1

Eluate 1a

1.2

2.4

4.9

0.9

5.2

4.7

4.9

Eluate 2a

1.7

3.3

6.6

0.3

20.1

6.5

18.8

d-Carb

Crude extract

128.9

257.8

1031.0

61.4

16.8

Eluate 1a

113.8

227.6

455.3

0.8

554.9

44.2

33.0

Eluate 2a

59.9

119.7

239.4

0.8

300.0

23.3

17.9

  1. aEluate 1 and 2: subsequent elutions of the same pulldown