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Table 1 Purification of the d-Hyd(co) and d-Carb via Ni Sepharose beads

From: Toward a cell-free hydantoinase process: screening for expression optimization and one-step purification as well as immobilization of hydantoinase and carbamoylase

Enzyme Fraction A (mU/mL) Avol (mU/mL) Atot (mU) cprot, tot (mg) Aspez (mU/mg) Recovery (%) Purification fold
d-Hyd(co) Crude extract 12.8 25.7 102.9 95.9 1.1
Eluate 1a 1.2 2.4 4.9 0.9 5.2 4.7 4.9
Eluate 2a 1.7 3.3 6.6 0.3 20.1 6.5 18.8
d-Carb Crude extract 128.9 257.8 1031.0 61.4 16.8
Eluate 1a 113.8 227.6 455.3 0.8 554.9 44.2 33.0
Eluate 2a 59.9 119.7 239.4 0.8 300.0 23.3 17.9
  1. aEluate 1 and 2: subsequent elutions of the same pulldown