Fig. 7From: Toward a cell-free hydantoinase process: screening for expression optimization and one-step purification as well as immobilization of hydantoinase and carbamoylaseSDS-PAGE analysis of different fractions after purification of both enzymes. a Ni Sepharose beads 1–5 hydantoinase, 1 insoluble fraction, 2 protein standard with molecular weights in kDa, 3 crude extract, 4 eluate 1, 5 eluate 2, 6 - 10 carbamoylase, 6 insoluble fraction, 7 crude extract, 8 protein standard with molecular weights in kDa, 9 eluate 1, 10 eluate 2. b Functionalized magnetic beads, 1–5 hydantoinase, 1 insoluble fraction, 2 protein standard with molecular weights in kDa, 3 crude cell extract, 4 wash 1, 5 eluate 6–10 carbamoylase, 6 protein standard with molecular weights in kDa, 7 insoluble fraction, 8 crude cell extract, 9 wash 1, 10 eluateBack to article page