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AMB Express
Fig. 2 | AMB Express

Fig. 2

From: Toward a cell-free hydantoinase process: screening for expression optimization and one-step purification as well as immobilization of hydantoinase and carbamoylase

Fig. 2

Cultivation of E. coli BL21DE3 and expression of d-Hyd and d-Hyd(co) under oxygen deficiency. a OD600 values of the d-Hyd and d-Hyd(co) cultures at the moment of harvesting after cultivation at 120 rpm (dark grey), 100 rpm (grey) and 90 rpm (white). b Specific activities of d-Hyd and d-Hyd(co) during whole cell biotransformations with 2 mM PheHyd as as substrate. The reactions were carried out at 40 °C and 800 rpm in Tris–HCl (50 mM, pH 8)

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