Table 2 The five criteria for selecting the amino acid positions for mutagenesis

B factor >25 Å²

K4, Q14, R17, E21, A24, P25, M26, E28, S36, P37, L38, D39, P40, A41, G42, P43, N44, E45, E46, Q49, A63, P79, E81, H101, G134, F154, C166, T167, P168, E169, N171, F180, S181, E190, H218, G223, K227, R235, H236, T238, E239, T240, T241, P242, P243, P244

Location

K4, M26, P37, L38, D39, P40, A41, G42, P43, N44, E45, A63, P79, E81, H101, T167, P168, E169, N171, F180, E190, K227, H236, T238, E239, P242, P243, P244

Occurrence in thermostable proteins

K4, M26, L38, D39, A41, G42, N44, E45, A63, E81, H101, T167, E169, N171, F180, E190, K227, H236, T238, E239

Conservation

M26, L38, D39, A41, G42, N44, A63, E81, H101, E169, N171, F180, E190, K227, H236, T238, E239

Part of hydrogen bonding network

L38, A41, E81, H101, H236, E239