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Table 2 The five criteria for selecting the amino acid positions for mutagenesis

From: Thermostabilization of the uronate dehydrogenase from Agrobacterium tumefaciens by semi-rational design

Criterion Amino acids
B factor >25 Å2 K4, Q14, R17, E21, A24, P25, M26, E28, S36, P37, L38, D39, P40, A41, G42, P43, N44, E45, E46, Q49, A63, P79, E81, H101, G134, F154, C166, T167, P168, E169, N171, F180, S181, E190, H218, G223, K227, R235, H236, T238, E239, T240, T241, P242, P243, P244
Location K4, M26, P37, L38, D39, P40, A41, G42, P43, N44, E45, A63, P79, E81, H101, T167, P168, E169, N171, F180, E190, K227, H236, T238, E239, P242, P243, P244
Occurrence in thermostable proteins K4, M26, L38, D39, A41, G42, N44, E45, A63, E81, H101, T167, E169, N171, F180, E190, K227, H236, T238, E239
Conservation M26, L38, D39, A41, G42, N44, A63, E81, H101, E169, N171, F180, E190, K227, H236, T238, E239
Part of hydrogen bonding network L38, A41, E81, H101, H236, E239