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Table 1 Primers used for saturation mutagenesis

From: Thermostabilization of the uronate dehydrogenase from Agrobacterium tumefaciens by semi-rational design

  Sequence 5′–3′
L38fw GATCTGTCTCCGNNKGATCCGGCTGGTCCGAATGAAG
L38rv CAGCCGGATCMNNCGGAGACAGATCTGCCAGAC
A41fw CCGCTGGATCCGNNKGGTCCGAATGAAGAATGTGTTC
A41rv CTTCATTCGGACCMNNCGGATCCAGCGGAGACAGATC
E81fw GCGTTGAAAAACCGTTTNNKCAGATTCTGCAGGGTAACATTATTGGC
E81rv CCCTGCAGAATCTGMNNAAACGGTTTTTCAACGCTAATGCCAC
H101fw GCAGCACGTGCANNKGGTCAGCCTCGTATTGTTTTTGCAAG
H101rv CAATACGAGGCTGACCMNNTGCACGTGCTGCTTCATACAG
H236fw GCCTTTCGTCGTNNKATTACCGAAACCACACCGCCTCCG
H236rv GGTGTGGTTTCGGTAATMNNACGACGAAAGGCTTCTGCATTATCTTTCGG
E239fw GTCGTCATATTACCNNKACCACACCGCCTCCGGATCCGAATG
E239rv CCGGAGGCGGTGTGGTMNNGGTAATATGACGACGAAAGGCTTC