Table 1 Primers used for saturation mutagenesis
Sequence 5′–3′ | |
|---|---|
L38fw | GATCTGTCTCCGNNKGATCCGGCTGGTCCGAATGAAG |
L38rv | CAGCCGGATCMNNCGGAGACAGATCTGCCAGAC |
A41fw | CCGCTGGATCCGNNKGGTCCGAATGAAGAATGTGTTC |
A41rv | CTTCATTCGGACCMNNCGGATCCAGCGGAGACAGATC |
E81fw | GCGTTGAAAAACCGTTTNNKCAGATTCTGCAGGGTAACATTATTGGC |
E81rv | CCCTGCAGAATCTGMNNAAACGGTTTTTCAACGCTAATGCCAC |
H101fw | GCAGCACGTGCANNKGGTCAGCCTCGTATTGTTTTTGCAAG |
H101rv | CAATACGAGGCTGACCMNNTGCACGTGCTGCTTCATACAG |
H236fw | GCCTTTCGTCGTNNKATTACCGAAACCACACCGCCTCCG |
H236rv | GGTGTGGTTTCGGTAATMNNACGACGAAAGGCTTCTGCATTATCTTTCGG |
E239fw | GTCGTCATATTACCNNKACCACACCGCCTCCGGATCCGAATG |
E239rv | CCGGAGGCGGTGTGGTMNNGGTAATATGACGACGAAAGGCTTC |