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Fig. 4 | AMB Express

Fig. 4

From: Mannan endo-1,4-β-mannosidase from Kitasatospora sp. isolated in Indonesia and its potential for production of mannooligosaccharides from mannan polymers

Fig. 4

a SDS-PAGE and western blotting of recombinant β-mannanase (ManKs_4-555) expressed in S. lividans 1326; STD, molecular weight marker; lane 1, culture supernatant from S. lividans 1326 (wild type); lane 2, culture supernatant from S. lividans 1326/pUC702-pro; lane 3, culture supernatant from S. lividans 1326/pUC702-pro-ManKs-(His)6 before purification; lane 4, purified β-mannanase (ManKs_4-555). b Effect of pH on β-mannanase activity of ManKs_4-555 containing 0.5% (w/v) LBG. Activity was measured at 45 °C using the following buffers: acetate buffer (pH 4.0–5.0, filled diamond), MES buffer (pH 5.0–7.0, filled square), MOPS buffer (pH 7.0–8.5, filled triangle), and glycine–NaOH buffer (pH 8.5–10, filled circle). ManKs_4-555 activity at pH 6.5 was taken to be 100%. c Temperature dependence of ManKs_4-555. Activity was measured at 30–90 °C using 50 mM MES buffer (pH 6.5) containing 0.5% (w/v) LBG. β-Mannanase activity at 65 °C was taken to be 100%. d Thermal stability of ManKs_4-555. Activity was measured at 45 °C (circle), 50 °C (square), and 55 °C (diamond) using 50 mM MES buffer (pH 6.5) for a maximum of 120 min. β-Mannanase activity at the starting point was taken to be 100%

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