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Table 3 Comparison of parameters yields of the best and highest mutant strains for ethanol production with R-control, during anaerobic fermentation in the presence of 20 g glucose/L

From: A novel constructed SPT15 mutagenesis library of Saccharomyces cerevisiae by using gTME technique for enhanced ethanol production

Strainsa

Parameters

Final DCW (g/L)

Residual GLC (g/L)

Ethanol production (g/L)

Increase (%)

YEG ETHO g/GLC g

YEF ETHO g/CDW g

R-control

1.991

1.400

9.814

0.527

4.927

R-M20C1-P3

2.040

2.350

15.722

+60.3

0.891

7.706

R-M16C4-P3

2.014

1.900

15.471

+57.7

0.855

7.679

R-M4C1-P3

2.032

1.900

15.470

+57.7

0.855

7.616

R-M12C4-P3

2.071

1.750

15.322

+56.2

0.839

7.402

R-M18C4-P3

2.070

2.050

15.290

+55.9

0.852

7.387

R-M5C1-P3

1.981

1.700

15.211

+55.1

0.831

7.680

R-M3C49-P10

2.011

1.900

14.036

+43.1

0.786

7.029

R-M18C5-P10

2.040

2.150

14.008

+42.8

0.785

6.861

R-M17C4-P20

1.984

2.050

13.486

+37.5

0.756

6.811

R-M9C2-P20

2.012

2.200

13.464

+37.2

0.761

6.724

  1. P3: 3% of MnCl2, P10: 10% of MnCl2 and P20: 20% of MnCl2; that were used in the Ep-PCR reaction
  2. CDW dry cell weight, GLC glucose, YEG yield of ETHO g/glucose used g, YEF yield of ETHO g/final CDW g
  3. aIndicates to the highest mutant strains for ethanol production