Table 2 The oligonucleotide primers used
Primer names | Sequence (5′–3′)a |
|---|---|
SPT15-EcoRI-FW | CCGGAATTCATGGCCGATGAGGAACGTTTAAAGG |
SPT15-SalI-RV | CGCTAGGTCGACTCACATTTTTCTAAATTCACTTAGCACA |
TAF23-NheI-RV | ACTCGAGCTAGCCTAACGATAAAAGTCTGGGCGACCT |
TAF23-SalI-FW | CGCTAGGTCGACATGGATTTTGAGGAAGATTACGAT |
pYX212-FW | GGGCAGCATAATTTAGGAG |
pYX212-RV | AGGGATGTAT CGGTCAGTCA |
425-TT-BamHI-FW | CGCGGATCCATCCGTATGATGTGCCTGACTA |
426-TT-EcoRV-RV | AATAAGATATCAGGGAAGAAAGCGAAAGGAG |
(MAT-F) | AGTCACATCAAGATCGTTTATGG |
(MAT-a) | ACTCCACTTCAAGTAAGAGTTTG |
(MAT-alpha) | GCACGGAATATGGGACTACTTCG |