Fig. 5
a Growth curve for the R-control strain and b–e growth curves for the best mutant strains (R-M3C49-P10, R-M18C5-P10, R-M17C4-P20 and R-M9C2-P20) obtained by the Ep-PCR reaction by using 10 and 20% MnCl2 concentrations, respectively. These strains were inoculated into the YPDT medium at different levels of ethanol concentrations (
) 0% ETHO, (
) 1% ETHO, (
) 3% ETHO, (
) 5% ETHO and (
) 7% ETHO