Fig. 4
a Growth curve for the R-control strain and b–g growth curves for the best mutant strains (R-M20C1, R-M16C4, R-M4C1, R-M12C4, R-M18C4 and R-M5C1) obtained by the Ep-PCR reaction by using 3% MnCl2 concentration, respectively. These strains were inoculated into the YPDT medium at different levels of ethanol concentrations (
) 0% ETHO, (
) 1% ETHO, (
) 3% ETHO, (
) 5% ETHO and (
) 7% ETHO