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Table 2 The kinetic parameters of reAoXyn11A and its three variants

From: Improving the temperature characteristics and catalytic efficiency of a mesophilic xylanase from Aspergillus oryzae, AoXyn11A, by iterative mutagenesis based on in silico design

Recombinant xylanase

K m (mg mL−1)

k cat (s−1)

k cat/K m (mL mg−1 s−1)

Fold

reAoXyn11A

3.33 ± 0.2

954.1 ± 49

286.5

1.00

reAoXyn11AG21I

2.32 ± 0.1

1246.8 ± 67

537.4

1.88

reAoXyn11AY13F

3.47 ± 0.2

1137.5 ± 53

327.8

1.14

reAoXyn11AG21I–Y13F

2.08 ± 0.1

984.1 ± 40

473.1

1.65

  1. K m and k cat values were measured under the standard assay conditions, except birchwood xylan concentrations from 1.0 to 10 mg mL−1. Data were expressed as the mean ± standard deviation (SD) from three independent replicates
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