Improving the temperature characteristics and catalytic efficiency of a mesophilic xylanase from Aspergillus oryzae, AoXyn11A, by iterative mutagenesis based on in silico design

AMB Express

Table 1 The temperature characteristics of seven representative thermophilic GHF11 xylanases

Xylanase	GenBank no.	Identity (%)	T _opt (°C)	Thermostability	Reference
EvXyn11^TS	ACB87631	63.5	75	T _m = 101 °C	Dumon et al. (2008)
NFX	CAD48747	56.6	–	t ¹⁰⁰_1/2 = 28 min	Hakulinen et al. (2003)
Xyn11A	AHK22788	56.1	80	80 °C	Zhao et al. (2015)
Thxyn11A	AEH04392	54.7	70	t ⁷⁰_1/2 = 90 min	Zhang et al. (2012)
TLX	AAB94633	53.3	75	t ⁸⁵_1/2 > 30 min	Hakulinen et al. (2003)
XynS14	BAK09352	52.8	80	t ⁸⁰_1/2 = 120 min	Sriyapai et al. (2011)
DTX	AAC46361	52.6	85	–	Hakulinen et al. (2003)