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AMB Express
Fig. 7 | AMB Express

Fig. 7

From: Improving the temperature characteristics and catalytic efficiency of a mesophilic xylanase from Aspergillus oryzae, AoXyn11A, by iterative mutagenesis based on in silico design

Fig. 7

Analysis of the intramolecular interactions in AoXyn11AG21I–Y13F. a Nine extra hydrophobic interactions (Ile21-Val41, Tyr34-Val172, Tyr71-Leu72, Ala73-Ile87, Val74-Val172, Tyr102-Ile115, Tyr113-Ile115, Ile115-Leu162 and Trp157-Tyr160) and one additional salt bridge (Glu84-Arg120) were generated by substituting Gly21 with Ile. Three extra hydrophobic interactions (Phe13-Tyr8, Phe13-Tyr169 and Phe13-Ile171) were formed by mutation of Tyr13Phe. b, c Two large hydrophobic regions (Tyr34-Tyr71-Leu72-Ala73-Val74-Ile87-Val172 and Tyr102-Tyr113-Ile115-Trp157-Tyr160-Leu162) were illustrated in the locally magnified 3-D structures. d Three hydrophobic interactions (Phe13-Tyr8, Phe13-Tyr169 and Phe13-Ile171) were illustrated in the locally magnified 3-D structure

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