Autolysis of Pichia pastoris induced by cold

AMB Express

Table 3 Real-time PCR primers and probes used in this study

Primer or probe^a	Sequence (5′–3′)	Modification^b
Primer
eng-F (TaqMan MGB)	GTCGGTTGACGGGTTAATTGTG	None
eng-R (TaqMan MGB)	AGCCGCATAGTCGTAGTAAATCA	None
act1-F (TaqMan MGB)	TGTCTGAGCGTGGTTACACTTTT	None
act1-R (TaqMan MGB)	CAACGTAACAAAGCTTCTCCTTGAT	None
Probe
eng (TaqMan® probe)	CTGCCTTGGCAACTTG	FAM/MGB-NFQ
act1 (TaqMan® probe)	CACGGACGATTTCTCT	FAM/MGB-NFQ

FAM fluorophore 6-carboxyfluorescein, MGB minor groove binder, NFQ nonfluorescent quencher
^a F forward primer, R reverse primer, eng gene encoding a putative endo-β-1,3-glucanase, act1 gene encoding actin 1
^bReporter 1 dye/reporter 1 quencher