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Table 2 Primers used in this study

From: Autolysis of Pichia pastoris induced by cold

Primera Sequence (5′→3′)b Restriction enzyme Underlined sequencec
YL1-F GGGGtcgcgaGAATCTTCCTCAGAAGAGGAAT NruI Homologous Pcctα region corresponding to nucleotides (nt) 886842 to 886863
YL1-R GCGCggatccTCACCTCCTAGGAAAAGGGTAT BamHI Homologous Pcctα region corresponding to nt 887263 to 887284
YL2-F CCCCagatctCCCAAATGTCATTCTCTTCCAA BglII Homologous eng region corresponding to nt 802095 to 802116
YL2-R GCGCctcgagTTAAACGGAATTAGCCAGTCCT XhoI Homologous eng region corresponding to nt 805153 to 805132
YL3-F GCGCgtcgacTGAGTTTTAGCCTTAGACATGACT SalI Homologous aox1 TT region corresponding to nt 627 to 650
YL3-R GGGGcacgtgCCGCACAAACGAAGGTCTCA PmlI Homologous aox1 TT region corresponding to nt 970 to 957
YL4-F GGGGatttaaatCCCACACACCATAGCTTCAAA SwaI Homologous Ptef1 region corresponding to nt 971 to 993
YL4-R GGGGcacgtgTCTCCAGCTTGCAAATTAAAG PmlI Homologous cyc1 TT region corresponding to nt 2142 to 2122
YL5-F GGGGtcgcgaGTATATTGAGGAGCAGAACTAG NruI Homologous leu2 region corresponding to nt 86482 to 86503
YL5-R GGGGcacgtgCTTTTTCCGAACCTTACAGTAG PmlI Homologous leu2 region corresponding to nt 87943 to 87922
  1. a F forward primer, R reverse primer
  2. bLowercase letters indicates the restriction endonuclease recognized site denoted in the next column
  3. cNucleotide numbering has been taken according to the sequence depicted in the access number mentioned in the section of materials and methods