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Table 1 Sequences used in this study

From: Autolysis of Pichia pastoris induced by cold

Sequence Description Source Amplicon Reference
Pcctα Alpha subunit promoter from cytoplasmic chaperonin CCT S. cerevisiae YL1 Chromosome IV, Accession No. NC_001136, nt: 886842-887284
eng Gene encoding a putative endo-β-1,3-glucanase P. pastoris YL2 Chromosome 1, Accession No. NC_012963; GeneID: 8197918
aox1 TT aox1 transcription termination region P. pastoris YL3 Online catalog. pGAPZα A, B, and C. Pichia expression vectors for constitutive expression and purification of recombinant proteins (Invitrogen, CA, USA)
Ptef1 Eukaryotic constitutive promoter S. cerevisiae YL4
PEM7 Prokaryotic constitutive promoter Synthetic
ble Gene encoding a protein that confers resistance to zeocin S. hindustanus
cyc1 TT End 3′ of the gene cyc1 for transcription termination S. cerevisiae
leu2 Functional gene of the β-isopropylmalate dehydrogenase (IMDH) P. pastoris YL5 Chromosome 3, Accession No. NC_012965; GeneID: 8199325