Table 1 Sequences used in this study
Sequence | Description | Source | Amplicon | Reference |
|---|---|---|---|---|
Pcctα | Alpha subunit promoter from cytoplasmic chaperonin CCT | S. cerevisiae | YL1 | Chromosome IV, Accession No. NC_001136, nt: 886842-887284 |
eng | Gene encoding a putative endo-β-1,3-glucanase | P. pastoris | YL2 | Chromosome 1, Accession No. NC_012963; GeneID: 8197918 |
aox1 TT | aox1 transcription termination region | P. pastoris | YL3 | Online catalog. pGAPZα A, B, and C. Pichia expression vectors for constitutive expression and purification of recombinant proteins (Invitrogen, CA, USA) https://www.thermofisher.com/order/catalog/product/V20020 |
Ptef1 | Eukaryotic constitutive promoter | S. cerevisiae | YL4 | |
PEM7 | Prokaryotic constitutive promoter | Synthetic | ||
ble | Gene encoding a protein that confers resistance to zeocin | S. hindustanus | ||
cyc1 TT | End 3′ of the gene cyc1 for transcription termination | S. cerevisiae | ||
leu2 | Functional gene of the β-isopropylmalate dehydrogenase (IMDH) | P. pastoris | YL5 | Chromosome 3, Accession No. NC_012965; GeneID: 8199325 |