Fig. 2

Analysis by optical microscopy (100×) after staining with Coomassie blue R-250 of Pichia pastoris GS115 (control) and P. pastoris CL2 cells induced by cold-shock and after the entire treatment. a, c Control cells of P. pastoris GS115 at 12 h and 24 h in stage IV, respectivelly; b, d P. pastoris CL2 cells at 12 and 24 h in stage IV, respectively. Bar 15 µm