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TableĀ 2 Amino acid sequences of bacterial and novel signal peptides used in this study

From: Novel signal peptides improve the secretion of recombinant Staphylococcus aureus Alpha toxinH35L in Escherichia coli

Plasmid ID Signal peptide ID The n-regiona The h-regionb The c-regionc
DsbAss_ATH35L DsbAss MKKI (+2) WLALAGLVL AFSASA
PelBss_ATH35L PelBss MKYLLP (+1) TAAAGLLLLA AQPAMA
PhoAss_ATH35L PhoAss MKQST (+1) IALALLPLL FTPVTKA
NTss_ATH35L NTssd MKTH (+1.1) IVSSVTTTLLLGSILMN PVANA
149153 NSP1 MKYLLP (+1) WLALAGLVL AFSASA
149154 NSP2 MKKI (+2) TAAAGLLLLA AFSASA
149155 NSP3 MKKI (+2) W1L2A3L4A5G6L7V8L9 AQPAMA
182988 NSP3a MKKI (+2) L9V8L7G6A5L4A3L2W1 AQPAMA
182989 NSP3b MKKI (+2) W1L2A3L7V8L9L4A5G6 AQPAMA
182990 NSP3c MKKI (+2) L4A5G6W1L2A3L7V8L9 AQPAMA
182991 NSP3d MKKI (+2) L7V8L9L4A5G6W1L2A3 AQPAMA
149156 NSP4 MKKI (+2) T1A2A3A4G5L6L7L8L9A10 AQPAMA
187441 NSP4a MKKI (+2) L6L7L8L9G5T1A2A3A4A10 AQPAMA
187442 NSP4b MKKI (+2) LLLLLLLLLL AQPAMA
187443 NSP4c MKKI (+2) AAAAAAAAAA AQPAMA
149157 NSP5 MKYLLP (+1) WLALAGLVL AQPAMA
149158 NSP6 MKYLLP (+1) TAAAGLLLLA AFSASA
  1. ss stands for signal sequence
  2. aNumbers in parentheses indicate the positive net charge in the n-region
  3. bNumbers indicate the position of amino acids in the h-region
  4. cThe cleavage sites are underlined in the c-region
  5. dAT native signal sequence