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Table 2 Amino acid sequences of bacterial and novel signal peptides used in this study

From: Novel signal peptides improve the secretion of recombinant Staphylococcus aureus Alpha toxinH35L in Escherichia coli

Plasmid ID

Signal peptide ID

The n-regiona

The h-regionb

The c-regionc

DsbAss_ATH35L

DsbAss

MKKI (+2)

WLALAGLVL

AFSASA

PelBss_ATH35L

PelBss

MKYLLP (+1)

TAAAGLLLLA

AQPAMA

PhoAss_ATH35L

PhoAss

MKQST (+1)

IALALLPLL

FTPVTKA

NTss_ATH35L

NTssd

MKTH (+1.1)

IVSSVTTTLLLGSILMN

PVANA

149153

NSP1

MKYLLP (+1)

WLALAGLVL

AFSASA

149154

NSP2

MKKI (+2)

TAAAGLLLLA

AFSASA

149155

NSP3

MKKI (+2)

W1L2A3L4A5G6L7V8L9

AQPAMA

182988

NSP3a

MKKI (+2)

L9V8L7G6A5L4A3L2W1

AQPAMA

182989

NSP3b

MKKI (+2)

W1L2A3L7V8L9L4A5G6

AQPAMA

182990

NSP3c

MKKI (+2)

L4A5G6W1L2A3L7V8L9

AQPAMA

182991

NSP3d

MKKI (+2)

L7V8L9L4A5G6W1L2A3

AQPAMA

149156

NSP4

MKKI (+2)

T1A2A3A4G5L6L7L8L9A10

AQPAMA

187441

NSP4a

MKKI (+2)

L6L7L8L9G5T1A2A3A4A10

AQPAMA

187442

NSP4b

MKKI (+2)

LLLLLLLLLL

AQPAMA

187443

NSP4c

MKKI (+2)

AAAAAAAAAA

AQPAMA

149157

NSP5

MKYLLP (+1)

WLALAGLVL

AQPAMA

149158

NSP6

MKYLLP (+1)

TAAAGLLLLA

AFSASA

  1. ss stands for signal sequence
  2. aNumbers in parentheses indicate the positive net charge in the n-region
  3. bNumbers indicate the position of amino acids in the h-region
  4. cThe cleavage sites are underlined in the c-region
  5. dAT native signal sequence