Fig. 2
Schematic representation of the novel signal peptide constructions used to express secreted ATH35L. a DsbAss and pelBss used as parental signal peptides. D–N, D–H and D–C represent the n-, h- and c-regions of dsbAss, respectively and P–N, P–H and P–C represent the n-, h- and c-regions of pelBss, respectively. b Set I novel signal peptides, NSP1–NSP6, were created by shuffling the n-, h- and c-regions from dsbAss and pelBss. c Set II novel signal peptides. NSP4a–NSP4c were created by modifying the h-region of NSP4 by changing the position of amino acid residues or substituting residues with polyleucine or polyalanine. d Set III novel signal peptides. NSP3a–NSP3d were created by modifying the position of amino acid residues in the h-region of NSP3. In c, d Numbers next to individual amino acids in the h-region indicate the original position in the h-region in (a)