Fig. 1From: Novel signal peptides improve the secretion of recombinant Staphylococcus aureus Alpha toxinH35L in Escherichia coli Western blot analysis of cell culture medium from ATH35L signal sequence variants in a Micro24 fed-batch process. Arrow indicates released mature ATH35L protein from live cells and asterisk indicates leaked ATH35L precursor from dead cells. Purified ATH35L protein was used as a reference. In the table, the levels of secreted ATH35L protein in culture medium were quantified by the band intensity of ATH35L from SDS-PAGE gel using the Image J 1.50i (Schneider et al. 2012)Back to article page