Cloning and characterization of the Type I Baeyer–Villiger monooxygenase from Leptospira biflexa

AMB Express

Table 4 Biooxidation of fused cyclobutanones mediated by BVMO_Lepto

No	BVMO Leptospira biflexa		Reference biotransformation using CHMO Acinetobacter sp. NCIMB 9871 as biocatalyst
	Conv^a (%)	ee _N, ee _ABN (%)^b	Yield (%)	ee _N, ee _ABN (%)^b	References
	N:ABN (%)	ee _N, ee _ABN (%)^b	N:ABN (%)	ee _N, ee _ABN (%)^b	References
34	94	50(−), 23(−)	74	95(−), >99(−)	Mihovilovic et al. (2005b)
34	50:50	50(−), 23(−)	51:49	95(−), >99(−)	Mihovilovic et al. (2005b)
35	8	44(−), 95(−)	80	60(−), >95(−)	Alphand and Furstoss (1992)
35	27:73	44(−), 95(−)	65:35	60(−), >95(−)	Alphand and Furstoss (1992)
36	47	96(nd), >99(nd)	84	44(nd), >99(nd)	Mihovilovic et al. (2008)
36	19:81	96(nd), >99(nd)	70:30	44(nd), >99(nd)	Mihovilovic et al. (2008)

N:ABN ratio of normal:abnormal lactones, nd not determined
^aRelative conversion (Conv) of starting material to lactone as determined by chiral phase GC
^bEnantiomeric excess for normal lactone (ee _N) and abnormal lactone (ee _ABN). The sign of specific rotation is indicated in brackets and was assigned according to the literature for reference biotransformations