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Table 4 Effects of yhaM deletion on the production of cysteine

From: Cysteine degradation gene yhaM, encoding cysteine desulfidase, serves as a genetic engineering target to improve cysteine production in Escherichia coli

Strain

Plasmid

Cysteine (mg L−1)

Yield (%)

OD660

MG1655 (WT)

pMIV-cysE5

64.6 ± 4.5

0.164 ± 0.011

28.6 ± 0.8

MG1655 ΔyhaM

pMIV-cysE5

316.2 ± 74.4

0.810 ± 0.195

29.4 ± 0.9

  1. Values represent the averages based on four independent experiments and the error value represents one standard deviation. To retain the plasmids, 25 μg mL−1 chloramphenicol was added. Cultivation was terminated at 15–17 h after inoculation when >95% of the total sugar (>38 g L−1) had been consumed
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