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Table 3 Effects of yhaM overexpression on the production of cysteine

From: Cysteine degradation gene yhaM, encoding cysteine desulfidase, serves as a genetic engineering target to improve cysteine production in Escherichia coli

Strain Plasmid Cysteine (mg L−1) Yield (%) OD660
MG1655 (WT) pMIV-cysE5, pACYC177 74.6 ± 7.0 0.187 ± 0.018 21.7 ± 0.8
pMIV-cysE5, pACYC-yhaM1 14.9 ± 1.1 0.037 ± 0.003 22.0 ± 0.8
  1. Values represent the averages based on five independent experiments and the error value represents one standard deviation. To retain the plasmids, 20 μg mL−1 kanamycin and 25 μg mL−1 chloramphenicol were added. Cultivation was terminated at 21–24 h after inoculation when all the sugar (40 g L−1) had been consumed