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Table 1 Experiment design and results of optimization of nutrient medium for the production of secondary metabolites from Streptomyces 1123 by the central composite design

From: Application of response surface methodology for optimization of medium components for the production of secondary metabolites by Streptomyces diastatochromogenes KX852460

Run no.

X1

X2

X3

X4

X5

Inhibition zone (mm)

Residual value

Observed

Predicted

1

4

4

0.4

0.4

0.04

30.5

32.0341

0.80684

2

8

8

0.8

0

0

18.9

20.1345

1.52224

3

4

12

0.4

0.4

0.04

20

21.3111

1.56492

4

4

−4

0.4

0.4

0.04

22

19.2044

1.56492

5

0

0

0

0

0.08

13.8

16.1961

1.52224

6

12

4

0.4

0.4

0.04

23

21.4044

1.56492

7

8

8

0

0.8

0

23

21.0378

1.52224

8

0

8

0.8

0

0.08

20.2

19.4878

1.52224

9

4

4

1.2

0.4

0.04

21

19.4811

1.56492

10

0

8

0.8

0.8

0

18.5

16.8995

1.52224

11

8

8

0

0

0.08

14

13.8661

1.52224

12

4

4

0.4

0.4

0.04

33.5

32.0341

0.80684

13

8

0

0

0.8

0.08

11

10.9545

1.52224

14

4

4

0.4

0.4

0.12

24.3

22.2777

1.56492

15

0

0

0.8

0.8

0.08

22.1

21.9161

1.52224

16

0

8

0

0

0

4.5

4.9795

1.52224

17

0

0

0

0.8

0

12

12.4078

1.52224

18

4

4

0.4

0.4

0.04

33.67

32.0341

0.80684

19

0

8

0

0.8

0.08

5.34

5.7511

1.52224

20

4

4

0.4

0.4

0.04

30.35

32.0341

0.80684

21

4

4

0.4

0.4

0.04

32.7

32.0341

0.80684

22

4

4

0.4

0.4

−0.04

18.5

19.0377

1.56492

23

8

0

0

0

0

2.0

1.8628

1.52224

24

−4

4

0.4

0.4

0.04

17.1

17.2111

1.56492

25

0

0

0.8

0

0

3.0

3.0645

1.52224

26

8

0

0.8

0.8

0

20.3

22.2228

1.52224

27

4

4

0.4

1.2

0.04

15

15.6944

1.56492

28

4

4

−0.4

0.4

0.04

8.43

8.4644

1.56492

29

4

4

0.4

0.4

0.04

30

32.0341

0.80684

30

8

8

0.8

0.8

0.08

10

11.1461

1.52224

31

8

0

0.8

0

0.08

13.6

16.2511

1.52224

32

4

4

0.4

−0.4

0.04

11.25

9.0711

1.56492

  1. Coded values; X 1  peanut meal, X 2  soluble starch, X 3  NaCl, X 4  yeast, X 5  ammonium sulphate