Table 1 Strains and plasmids used in this study
Strain or plasmid | Description | Reference |
|---|---|---|
Strains | ||
E. coli DH5α | F- Phi80lacZDeltaM15 Delta(lacZYA-argF) U169 recA1 endA1 hsdR17(rk−, mk +) phoA supE44 thi-1 gyrA96 relA1 tonA | Invitrogen |
E. coli D1210HP | F- recA56 thi-1 leu-6 proA2 hsdS20 (rB-mB-) lacY1 galK2 ara-14 mtl-1 xyl-5 lacIq lacY1 endA rpsL20 (Lambdaxis-kil-cI857) | Merck |
Plasmids | ||
pcDNA 3.1(+) | Vector for high-level expression in mammalian cells | Invitrogen |
pElysM | Highly efficient host-killing vector | Ma et al. (2009) |
pC3.1 | Derivative of pcDNA 3.1(+), lacking SV40 origin and neomycin selection gene | This study |
pC3.2 | Derivative of pC3.1, containing Lac operator, ribosome biding site (RBS), Kozak sequence and 6His tag | This study |
pC3.3 | Derivative of pC3.2, containing T7 terminator | This study |
pOmni | Derivative of pC3.3, containing lysis gene E expression cassette | This study |
pIRES2-EGFP | EGFP reporter expression vector | Clontech |