Fig. 2From: Development of a dual-expression vector facilitated with selection-free PCR recombination cloning strategyDetailed sequence information of designed functional elements in pOmni. Lac operator, ribosome biding site (RBS), Kozak sequence and 6His tag were introduced between T7 promoter and BGH Poly(A) site. PCR amplified target gene products containing two primer-introduced terminal sequences (shaded sequences) can be directionally and seamlessly inserted into correspondent position and replace the suicide gene through recombination PCR. The designed Kozak sequence and its proper position related to RBS make it possible to express cloned target gene in both E. coli and eukaryotic cellsBack to article page