Ammonia production from amino acid-based biomass-like sources by engineered Escherichia coli

AMB Express

Table 3 Ammonia production by the kivd + ΔglnA strain with various nitrogen sources (7.25 g/L)

Nitrogen source	Cell density OD₆₀₀	Produced ammonia by E. coli (mg/L)	Theoretical amount of maximum ammonia production (mg/L)^a	Yield (%)
Yeast extract (M9_YE)	1.51 ± 0.05	439 ± 12	960	45.8
Tryptone	1.25 ± 0.08	427 ± 25	1171	36.5
Peptone	1.00 ± 0.06	375 ± 10	1356	27.7
Casamino acids	0.99 ± 0.03	453 ± 35	951	47.6

Values of yield were calculated as produced ammonia by E. coli per theoretical amount of maximum ammonia production. Values given as mean ± SD (n = 3)
^aThe values were calculated based on the nitrogen contents of BD Bionutrients™ Technical Manual Third edition (http://www.bd.com/ds/technicalCenter/misc/lcn01558-bionutrients-manual.pdf.)