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Table 3 Ammonia production by the kivd + ΔglnA strain with various nitrogen sources (7.25 g/L)

From: Ammonia production from amino acid-based biomass-like sources by engineered Escherichia coli

Nitrogen source Cell density OD600 Produced ammonia by E. coli (mg/L) Theoretical amount of maximum ammonia production (mg/L)a Yield (%)
Yeast extract (M9_YE) 1.51 ± 0.05 439 ± 12 960 45.8
Tryptone 1.25 ± 0.08 427 ± 25 1171 36.5
Peptone 1.00 ± 0.06 375 ± 10 1356 27.7
Casamino acids 0.99 ± 0.03 453 ± 35 951 47.6
  1. Values of yield were calculated as produced ammonia by E. coli per theoretical amount of maximum ammonia production. Values given as mean ± SD (n = 3)
  2. aThe values were calculated based on the nitrogen contents of BD Bionutrients™ Technical Manual Third edition (http://www.bd.com/ds/technicalCenter/misc/lcn01558-bionutrients-manual.pdf.)