Ammonia production from amino acid-based biomass-like sources by engineered Escherichia coli

AMB Express

Table 2 Growth and ammonia production of decarboxylase-overexpressing E. coli strains in M9_YE medium

Sample	Cell density OD₆₀₀	Produced ammonia by E. coli (mg/L)	Theoretical amount of maximum ammonia production (mg/L)^a	Yield (%)
Medium only	0.0253 ± 0.0073	24.2 ± 2.7	960	2.50
Control strain	3.73 ± 0.05	260 ± 11	960	27.1
kivd	2.31 ± 0.09	351 ± 5	960	36.6
cadA	5.11 ± 0.03	230 ± 6	960	24.0
gadA	2.89 ± 0.01	256 ± 6	960	26.7
ilvH	5.61 ± 0.07	239 ± 26	960	24.9

Values of yield were calculated as produced ammonia by E. coli per theoretical amount of maximum ammonia production. Values given as mean ± SD (n = 3)
^aThe values were calculated based on the nitrogen contents of BD Bionutrients™ Technical Manual Third edition (http://www.bd.com/ds/technicalCenter/misc/lcn01558-bionutrients-manual.pdf.)