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Table 2 Growth and ammonia production of decarboxylase-overexpressing E. coli strains in M9_YE medium

From: Ammonia production from amino acid-based biomass-like sources by engineered Escherichia coli

Sample Cell density OD600 Produced ammonia by E. coli (mg/L) Theoretical amount of maximum ammonia production (mg/L)a Yield (%)
Medium only 0.0253 ± 0.0073 24.2 ± 2.7 960 2.50
Control strain 3.73 ± 0.05 260 ± 11 960 27.1
kivd 2.31 ± 0.09 351 ± 5 960 36.6
cadA 5.11 ± 0.03 230 ± 6 960 24.0
gadA 2.89 ± 0.01 256 ± 6 960 26.7
ilvH 5.61 ± 0.07 239 ± 26 960 24.9
  1. Values of yield were calculated as produced ammonia by E. coli per theoretical amount of maximum ammonia production. Values given as mean ± SD (n = 3)
  2. aThe values were calculated based on the nitrogen contents of BD Bionutrients™ Technical Manual Third edition (http://www.bd.com/ds/technicalCenter/misc/lcn01558-bionutrients-manual.pdf.)