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Table 1 Strains and plasmids

From: Ammonia production from amino acid-based biomass-like sources by engineered Escherichia coli

Strains or plasmids

Genotype or description

Reference

E. coli strains

 DH10B

F–mcrA Δ(mrr-hsdRMS-mcrBC) Ф80lacZΔM15ΔlacX74recA1endA1araD139 Δ(ara leu) 7697galUgalKrpsLnupG λ–

Thermo Fisher Scientific

 DH10BΔglnA

DH10B ΔglnA:: FRT-kan R-FRT

This study

 DH10BΔgdhA

DH10B ΔgdhA:: FRT-kan R-FRT

This study

Plasmids

 pTrcHis2-TOPO

Ptrc:: TOPO site, pBR322 ori, lacI q, Amp R

Thermo Fisher Scientific

 Control plasmid (pTrcHis2-lacZ)

Ptrc:: lacZ, pBR322 ori, lacI q, Amp R

Thermo Fisher Scientific

 pTrcHis2-kivd

Ptrc:: kivd, pBR322 ori, lacI q, Amp R

This study, Accession number: AJ746364.1

 pTrcHis2-cadA

Ptrc:: cadA, pBR322 ori, lacI q, Amp R

This study, Accession number: AY319765.1

 pTrcHis2-gadA

Ptrc:: gadA, pBR322 ori, lacI q, Amp R

This study, Accession number: EF547388.1

 pTrcHis2-ilvH

Ptrc:: ilvH, pBR322 ori, lacI q, Amp R

This study, Accession number: EG10499

 pKD46

Red recombinase expression plasmid

Datsenko et al. (2000)

 pKD13

Kan R template plasmid

Datsenko et al. (2000)