From: Ammonia production from amino acid-based biomass-like sources by engineered Escherichia coli
Strains or plasmids | Genotype or description | Reference |
---|---|---|
E. coli strains | ||
DH10B | F–mcrA Δ(mrr-hsdRMS-mcrBC) Ф80lacZΔM15ΔlacX74recA1endA1araD139 Δ(ara leu) 7697galUgalKrpsLnupG λ– | Thermo Fisher Scientific |
DH10BΔglnA | DH10B ΔglnA:: FRT-kan R-FRT | This study |
DH10BΔgdhA | DH10B ΔgdhA:: FRT-kan R-FRT | This study |
Plasmids | ||
pTrcHis2-TOPO | Ptrc:: TOPO site, pBR322 ori, lacI q, Amp R | Thermo Fisher Scientific |
Control plasmid (pTrcHis2-lacZ) | Ptrc:: lacZ, pBR322 ori, lacI q, Amp R | Thermo Fisher Scientific |
pTrcHis2-kivd | Ptrc:: kivd, pBR322 ori, lacI q, Amp R | This study, Accession number: AJ746364.1 |
pTrcHis2-cadA | Ptrc:: cadA, pBR322 ori, lacI q, Amp R | This study, Accession number: AY319765.1 |
pTrcHis2-gadA | Ptrc:: gadA, pBR322 ori, lacI q, Amp R | This study, Accession number: EF547388.1 |
pTrcHis2-ilvH | Ptrc:: ilvH, pBR322 ori, lacI q, Amp R | This study, Accession number: EG10499 |
pKD46 | Red recombinase expression plasmid | Datsenko et al. (2000) |
pKD13 | Kan R template plasmid | Datsenko et al. (2000) |