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Table 1 Strains and plasmids

From: Ammonia production from amino acid-based biomass-like sources by engineered Escherichia coli

Strains or plasmids Genotype or description Reference
E. coli strains
 DH10B F–mcrA Δ(mrr-hsdRMS-mcrBC) Ф80lacZΔM15ΔlacX74recA1endA1araD139 Δ(ara leu) 7697galUgalKrpsLnupG λ– Thermo Fisher Scientific
 DH10BΔglnA DH10B ΔglnA:: FRT-kan R-FRT This study
 DH10BΔgdhA DH10B ΔgdhA:: FRT-kan R-FRT This study
Plasmids
 pTrcHis2-TOPO Ptrc:: TOPO site, pBR322 ori, lacI q, Amp R Thermo Fisher Scientific
 Control plasmid (pTrcHis2-lacZ) Ptrc:: lacZ, pBR322 ori, lacI q, Amp R Thermo Fisher Scientific
 pTrcHis2-kivd Ptrc:: kivd, pBR322 ori, lacI q, Amp R This study, Accession number: AJ746364.1
 pTrcHis2-cadA Ptrc:: cadA, pBR322 ori, lacI q, Amp R This study, Accession number: AY319765.1
 pTrcHis2-gadA Ptrc:: gadA, pBR322 ori, lacI q, Amp R This study, Accession number: EF547388.1
 pTrcHis2-ilvH Ptrc:: ilvH, pBR322 ori, lacI q, Amp R This study, Accession number: EG10499
 pKD46 Red recombinase expression plasmid Datsenko et al. (2000)
 pKD13 Kan R template plasmid Datsenko et al. (2000)