Growth kinetics, fatty acid composition and metabolic activity changes of Crypthecodinium cohnii under different nitrogen source and concentration

Safdar, Waseem; Shamoon, Muhammad; Zan, Xinyi; Haider, Junaid; Sharif, Hafiz Rizwan; Shoaib, Muhammad; Song, Yuanda

doi:10.1186/s13568-017-0384-3

Table 4 Comparison of the activity of key enzymes (nmol/min mg protein) related to lipid accumulation in C. Cohnii at three growth stages under different N-sources

From: Growth kinetics, fatty acid composition and metabolic activity changes of Crypthecodinium cohnii under different nitrogen source and concentration

NaNO₃ conc.	Growth phase	Activity of key enzymes (nmol/min mg protein)
NaNO₃ conc.	Growth phase	ME	ACL	FAS	G6PD	NADP-ICDH	CS	NAD-ICDH
0.2 g/L	Growth	58.7 ± 4.7	47.5 ± 4.7	152.1 ± 1.8	552.4 ± 1.5	168.4 ± 3.7	1483.8 ± 41.8	121.5 ± 2.9
	Lipid accumulation	59.1 ± 7.2	85.9 ± 2.8	168.2 ± 5.7	638.5 ± 2.4	87.7 ± 6.8	1428.1 ± 22.5	58.1 ± 1.8
	Lipid turnover	29.5 ± 3.9	43.6 ± 3.9	128.3 ± 9.5	413.1 ± 3.7	71.6 ± 2.9	1344.5 ± 13.9	53.9 ± 2.5
0.4 g/L	Growth	56.9 ± 6.5	40.5 ± 2.5	141.9 ± 2.1	469.4 ± 1.2	253.9 ± 2.71	1491.4 ± 22.5	127.5 ± 3.6
	Lipid accumulation	41.7 ± 7.5	72.8 ± 3.1	153.5 ± 5.9	461.9 ± 3.6	168.6 ± 8.9	1325.7 ± 21.7	82.8 ± 1.5
	Lipid turnover	29.4 ± 4.7	36.6 ± 4.5	139.2 ± 5.6	331.8 ± 3.8	159.1 ± 3.4	1258.2 ± 12.3	103.4 ± 1.5
0.8 g/L	Growth	47.8 ± 8.5	30.8 ± 2.4	146.8 ± 3.9	421.8 ± 1.8	264.9 ± 4.9	1505.9 ± 13.1	135.3 ± 2.8
	Lipid accumulation	35.9 ± 5.4	62.1 ± 1.9	146.9 ± 4.2	471.5 ± 1.2	269.5 ± 9.5	1381.3 ± 21.7	129.7 ± 1.5
	Lipid turnover	23.2 ± 2.9	38.5 ± 1.9	125.2 ± 6.3	398.1 ± 1.6	266.2 ± 1.6	1350.5 ± 31.4	126.2 ± 4.7
1.2 g/L	Growth	39.9 ± 2.6	28.9 ± 3.5	139.5 ± 2.1	413.6 ± 3.1	253.9 ± 2.6	1479.2 ± 25.5	131.5 ± 3.9
	Lipid accumulation	31.7 ± 4.7	56.2 ± 7.4	141.5 ± 2.5	385.9 ± 2.6	168.6 ± 2.5	1415.5 ± 42.2	129.8 ± 1.9
	Lipid turnover	27.5 ± 1.2	33.5 ± 5.2	133.9 ± 5.5	372.4 ± 1.4	159.1 ± 10.8	1378.4 ± 23.2	127.9 ± 4.5
1.6 g/L	Growth	38.5 ± 2.8	26.5 ± 2.8	132.7 ± 2.5	292.7 ± 1.8	253.9 ± 1.8	1461.4 ± 31.5	128.6 ± 2.5
	Lipid accumulation	33.8 ± 3.9	51.1 ± 2.4	127.9 ± 1.8	264.1 ± 2.5	168.6 ± 8.7	1409.7 ± 23.3	117.1 ± 2.5
	Lipid turnover	27.1 ± 6.5	24.9 ± 2.71	130.5 ± 1.6	227.5 ± 3.2	159.1 ± 3.7	1388.5 ± 12.5	117.9 ± 3.8

All experiments were performed in triplicate. The data presented here is mean ± SD
G6PD glucose-6-phosphate dehydrogenase, CS citrate synthase, NAD ⁺ -ICDH NAD dependent isocitrate dehydrogenase, NADP ⁺ -ICDH NADP dependent isocitrate dehydrogenase, ACL ATP-citrate lyase, ME malic enzyme, FAS fatty acid synthase

Back to article page