Table 3 Comparison of the activity of key enzymes (nmol/min mg protein) related to lipid accumulation in C. Cohnii at three growth stages under different N-sources
N-sources | Growth stages | Activity of key enzymes (nmol/min mg protein) | ||||||
|---|---|---|---|---|---|---|---|---|
FAS | ME | ACL | G6PD | NADP-ICDH | CS | NAD-ICDH | ||
(NH4)2SO4 | Growth | 129.1 ± 4.2 | 40.5 ± 2.9 | 27.9 ± 6.2 | 252.1 ± 3.7 | 498.1 ± 1.9 | 1613.4 ± 21.6 | 248.4 ± 2.1 |
Lipid accumulation | 138.6 ± 1.9 | 22.5 ± 6.5 | 52.2 ± 7.3 | 238.9 ± 1.6 | 417.3 ± 3.7 | 1428.1 ± 13.2 | 235.9 ± 15 | |
Lipid turnover | 125.9 ± 3.8 | 17.9 ± 2.4 | 18.6 ± 1.7 | 113.7 ± 6.1 | 331.6 ± 1.6 | 1341.7 ± 22.7 | 218.5 ± 2.2 | |
NH4 HCO3 | Growth | 127.6 ± 6.1 | 41.5 ± 4.1 | 26.5 ± 2.5 | 253.7 ± 2.4 | 495.9 ± 3.9 | 1602.5 ± 31.8 | 247.5 ± 2.5 |
Lipid accumulation | 138.2 ± 2.4 | 24.7 ± 3.8 | 52.4 ± 1.8 | 242.1 ± 1.8 | 407.2 ± 5.2 | 1398.7 ± 21.9 | 231.7 ± 5.2 | |
Lipid turnover | 125.5 ± 7.2 | 17.2 ± 3.4 | 19.1 ± 3.2 | 113.2 ± 2.5 | 328.6 ± 1.5 | 1356.2 ± 43.5 | 213.6 ± 9.2 | |
(NH2)2CO | Growth | 140.2 ± 2.7 | 45.7 ± 3.6 | 34.4 ± 1.9 | 241.5 ± 2.8 | 382.4 ± 7.4 | 1705.5 ± 23.38 | 198.5 ± 1.5 |
Lipid accumulation | 146.7 ± 11.9 | 34.2 ± 5.2 | 59.1 ± 2.8 | 239.3 ± 4.6 | 349.5 ± 1.8 | 1581.6 ± 32.8 | 194.6 ± 3.5 | |
Lipid turnover | 130.9 ± 4.05 | 21.5 ± 1.9 | 22.2 ± 1.6 | 207.5 ± 1.9 | 364.9 ± 2.6 | 1520.5 ± 45.2 | 189.1 ± 1.5 | |
NaNO3 | Growth | 141.9 ± 7.1 | 56.9 ± 6.5 | 40.5 ± 2.5 | 469.4 ± 1.2 | 253.9 ± 2.7 | 1491.4 ± 22.9 | 127.5 ± 3.1 |
Lipid accumulation | 153.5 ± 5.9 | 41.7 ± 7.5 | 72.8 ± 3.5 | 461.9 ± 3.6 | 168.6 ± 8.9 | 1325.7 ± 21.7 | 82.8 ± 1.5 | |
Lipid turnover | 139.2 ± 5.6 | 29.4 ± 4.7 | 36.6 ± 4.5 | 331.8 ± 3.8 | 159.1 ± 3.4 | 1258.2 ± 12.8 | 103.4 ± 1.5 | |