Fig. 2
From: Degradation and inactivation of Shiga toxins by nitrogen gas plasma
Quantitative measurement of Stx1 and Stx2 by an enzyme-linked immunosorbent assay (ELISA) after nitrogen gas plasma treatment. A coverslip containing dried spots from 20 μl aliquots of a 1 μg/ml solution of Stx1 (a) and Stx2 (b) was treated with nitrogen gas plasma using BLP-TES device (1.5 kpps) for 0, 5, 15, and 30 min. The recovered samples were subjected to ELISA using RIDASCREEN® Verotoxin (R-Biopharm AG, Darmstadt, Germany) to quantify Stx1 and Stx2. Values were considered significantly different from the untreated control (0 min) when verified by non-repeated measures ANOVA, followed by the Bonferroni correction (**p < 0.01)