Table 1 Oligonucleotide sequences for quantitative PCR
From: Quantitative detection of fecal contamination with domestic poultry feces in environments in China
Primer or probe | Oligonucleotide sequence (5′–3′) | Tm (°C) | Amplicon size (bp) |
|---|---|---|---|
Chicken and duck ND5-F | ACCTCCCCCAACTAGC | 53.6 | 172 |
Chicken and duck ND5-R | TTGCCAATGGTTAGGCAGGAG | 57.7 | Â |
Chicken and duck ND5-P | (FAM)TCAACCCATGCCTTCTT(NFQ-MGB) | 61.1 | Â |
Chicken and duck cytb-F | AAATCCCACCCCCTACTAAAAATAAT | 54.3 | 263 |
Chicken and duck cytb-R | CAGATGAAGAAGAATGAGGCG | 53.4 | Â |
Chicken and duck cytb-P | (FAM)ACAACTCCCTAATCGACCT(NFQ-MGB) | 62.7 | Â |