Fig. 2
From: Expressing anti-HIV VRC01 antibody using the murine IgG1 secretion signal in Pichia pastoris
Expression of VRC01 light and heavy chain in P. pastoris determined by dot blot. Dot blot was used to confirm the expression of the VRC01 from culture supernatants using a Rabbit Anti-Human IgG heavy and light antibody conjugated to alkaline phosphatase. 1–6 culture supernatants; a–c—α-MF strains, d–f—murine IgG1 strains. 7a Δku70, 7b–f blank