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Table 1 Fermentative parameters of lab-scale vinification assays in 20°Bx white must by parental and mutant laboratory yeast strains

From: Construction of low-ethanol–wine yeasts through partial deletion of the Saccharomyces cerevisiae PDC2 gene

Parameter

BY4741

BY4741

pdc2Δ519

BY4743

BY4743

pdc2Δ519

BY4741

BY4741

pdc2Δ344

BY4743

BY4743

pdc2Δ344

BY4743

pdc2Δ519

Main compounds (g/Liter)

V1

V1

V1

V1

V2

V2

V2

V2

V2

 Consumed sugar

153.95 ± 0.87A

146.49 ± 0.63B

147.22 ± 0.00B

143.84 ± 1.66C

172.04 ± 4.64B,C

154.90 ± 4.22D

179.56 ± 3.08A

170.98 ± 1.98C

176.05 ± 4.71A,B

 CO2

71.11 ± 1.92A

73.33 ± 5.77A

66.67 ± 0.00A,B

62.22 ± 3.85B

81.88 ± 3.89A,B

72.48 ± 2.52C

83.45 ± 1.60A

78.68 ± 2.84B

83.75 ± 2.20A

 Ethanol

75.61 ± 0.91A

75.35 ± 2.23A

74.56 ± 1.58A

69.04 ± 0.79B

87.58 ± 5.69A

76.007 ± 2.28C

87.74 ± 1.41A

84.27 ± 2.58A,B

81.27 ± 1.48B

 Acetate

1.11 ± 0.16A

0.91 ± 0.06A,B

0.88 ± 0.24A,B

0.78 ± 0.07B

1.11 ± 0.11A

0.63 ± 0.06B,C

0.58 ± 0.12C

0.83 ± 0.22B

0.60 ± 0.09C

 Glycerolb

ND

ND

ND

ND

2.67 ± 0.20B

2.44 ± 0.074B,C

2.58 ± 0.14B

3.16 ± 0.11A

2.24 ± 0.18C

Balance (%)a

         

 Carbon

97.18 ± 1.27B

101.43 ± 3.18A

97.64 ± 0.72A,B

95.26 ± 1.13B

99.32 ± 2.50A

97.33 ± 1.27A,B

96.40 ± 0.96A,B

96.67 ± 2.66A,B

95.18 ± 2.37B

Yield

         

 Ethanol production (%[v/v])

9.58 ± 0.12A

9.55 ± 0.20A

9.45 ± 0.20A

8.80 ± 0.10B

11.1 ± 0.72A

9.63 ± 0.29C

11.12 ± 0.18A

10.68 ± 0.33A,B

10.30 ± 0.19B

 EtOH (g/g glucose consumed)

0.49 ± 0.008A

0.51 ± 0.011A

0.51 ± 0.009A

0.48 ± 0.009A

0.51 ± 0.02A

0.49 ± 0.02A,B

0.49 ± 0.008B

0.49 ± 0.019A,B

0.46 ± 0.012C

 Glucose (g) required for 1% (v/v) ethanol production

16.07 ± 0.28A,B

15.34 ± 0.27C

15.58 ± 0.33B,C

16.35 ± 0.32A

15.52 ± 0.62B

16.09 ± 0.59B

16.15 ± 0.25B

16.02 ± 0.62B

17.09 ± 0.42A

 Glucose (g) required for 1 g/L glycerol

Residual sugar (g/Liter)

ND

62.65 ± 0.87C

ND

70.11 ± 0.63B

ND

69.38 ± 0.00B

ND

72.76 ± 1.66A

65.64 ± 4.21B

44.56 ± 4.64B,C

69.49 ± 2.30A,B

61.7 ± 4.22A

64.56 ± 3.83B

37.04 ± 3.08D

54.57 ± 1.99C

45.62 ± 1.98B

73.64 ± 3.38A

40.55 ± 4.71C,D

  1. ND not determined
  2. Distinct letters correspond to statistical significant difference for a Fischer test with p < 0.05
  3. aCarbon balance represents the ratio between carbon moles of fermentation by-products and carbon moles of glucose
  4. bGlycerol was measured in an independent experiment