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Table 4 Residual activity of Mrl2 towards ABTS in presence of different metal ions, cosolvents and inhibitors

From: Expression of a new laccase from Moniliophthora roreri at high levels in Pichia pastoris and its potential application in micropollutant degradation

Metal ions Residual activity (%) Cosolvent Residual activity (%) Inhibitor Concentration Residual activity (%)
10 mM 100 mM 10% 20%
Mn2+ 99.3 ± 1.9 119.4 ± 3.8 Ethanol 81.9 ± 1.5 21.7 ± 2.4 SDS 0.1% 100.3 ± 0.7
Co2+ 100.9 ± 0.9 104.6 ± 2.9 Methanol 76.5 ± 2.7 39 ± 0.8 1% 77.7 ± 1.1
Ni2+ 82.7 ± 1.5 26.2 ± 0.9 2-Propanol 61.9 ± 2.5 5.5 ± 2.5    
Ca2+ 85.4 ± 1 50.4 ± 1.9 ACN 52 ± 2.8 0.7 ± 0.4 Sodium azide 0.1% 3.3 ± 1.9
Cu2+ 104.9 ± 2.5 95.2 ± 2.7 DMSO 22.9 ± 2.2 26.1 ± 3.6    
Na+ 99 ± 2.8 105.1 ± 4.4 Acetone 22.2 ± 2 16.6 ± 2.3 Cl 10 mM 37.3 ± 0.9
Mg2+ 101.5 ± 1.5 80.1 ± 1.7 DMF 40.2 ± 4.3 16.6 ± 0.4 50 mM 12.2 ± 0.15
Zn2+ 104.9 ± 2.1 97.0 ± 1.2 Glycerol 85.7 ± 0.5 89.7 ± 1.9 100 mM 6.7 ± 0.04
  1. ACN acetonitrile, DMSO dimethyl sulfoxide, DMF dimethylformamid