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Table 4 Residual activity of Mrl2 towards ABTS in presence of different metal ions, cosolvents and inhibitors

From: Expression of a new laccase from Moniliophthora roreri at high levels in Pichia pastoris and its potential application in micropollutant degradation

Metal ions

Residual activity (%)

Cosolvent

Residual activity (%)

Inhibitor

Concentration

Residual activity (%)

10 mM

100 mM

10%

20%

Mn2+

99.3 ± 1.9

119.4 ± 3.8

Ethanol

81.9 ± 1.5

21.7 ± 2.4

SDS

0.1%

100.3 ± 0.7

Co2+

100.9 ± 0.9

104.6 ± 2.9

Methanol

76.5 ± 2.7

39 ± 0.8

1%

77.7 ± 1.1

Ni2+

82.7 ± 1.5

26.2 ± 0.9

2-Propanol

61.9 ± 2.5

5.5 ± 2.5

   

Ca2+

85.4 ± 1

50.4 ± 1.9

ACN

52 ± 2.8

0.7 ± 0.4

Sodium azide

0.1%

3.3 ± 1.9

Cu2+

104.9 ± 2.5

95.2 ± 2.7

DMSO

22.9 ± 2.2

26.1 ± 3.6

   

Na+

99 ± 2.8

105.1 ± 4.4

Acetone

22.2 ± 2

16.6 ± 2.3

Cl−

10 mM

37.3 ± 0.9

Mg2+

101.5 ± 1.5

80.1 ± 1.7

DMF

40.2 ± 4.3

16.6 ± 0.4

50 mM

12.2 ± 0.15

Zn2+

104.9 ± 2.1

97.0 ± 1.2

Glycerol

85.7 ± 0.5

89.7 ± 1.9

100 mM

6.7 ± 0.04

  1. ACN acetonitrile, DMSO dimethyl sulfoxide, DMF dimethylformamid