Expression of a new laccase from Moniliophthora roreri at high levels in Pichia pastoris and its potential application in micropollutant degradation

AMB Express

Table 4 Residual activity of Mrl2 towards ABTS in presence of different metal ions, cosolvents and inhibitors

Metal ions	Residual activity (%)		Cosolvent	Residual activity (%)		Inhibitor	Concentration	Residual activity (%)
Metal ions	10 mM	100 mM	Cosolvent	10%	20%	Inhibitor	Concentration	Residual activity (%)
Mn²⁺	99.3 ± 1.9	119.4 ± 3.8	Ethanol	81.9 ± 1.5	21.7 ± 2.4	SDS	0.1%	100.3 ± 0.7
Co²⁺	100.9 ± 0.9	104.6 ± 2.9	Methanol	76.5 ± 2.7	39 ± 0.8	SDS	1%	77.7 ± 1.1
Ni²⁺	82.7 ± 1.5	26.2 ± 0.9	2-Propanol	61.9 ± 2.5	5.5 ± 2.5
Ca²⁺	85.4 ± 1	50.4 ± 1.9	ACN	52 ± 2.8	0.7 ± 0.4	Sodium azide	0.1%	3.3 ± 1.9
Cu²⁺	104.9 ± 2.5	95.2 ± 2.7	DMSO	22.9 ± 2.2	26.1 ± 3.6
Na⁺	99 ± 2.8	105.1 ± 4.4	Acetone	22.2 ± 2	16.6 ± 2.3	Cl⁻	10 mM	37.3 ± 0.9
Mg²⁺	101.5 ± 1.5	80.1 ± 1.7	DMF	40.2 ± 4.3	16.6 ± 0.4		50 mM	12.2 ± 0.15
Zn²⁺	104.9 ± 2.1	97.0 ± 1.2	Glycerol	85.7 ± 0.5	89.7 ± 1.9		100 mM	6.7 ± 0.04