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Table 2 SEC-TDA analyses of polysaccharides produced by the recombinant strain of S. equi subsp. zooepidemicus-pNZ8148kfoAkfoC during growth in shakeflasks on semidefined medium

From: Engineering S. equi subsp. zooepidemicus towards concurrent production of hyaluronic acid and chondroitin biopolymers of biomedical interest

 

S. equi subsp. zooepidemicus-pNZ8148kfoAkfoC

S. equi subsp. zooepidemicus-pNZ8148kfoAkfoC + 0.5 mM GalNAc

S. equi subsp. zooepidemicus-pNZ8148kfoAkfoC + 2 mM phosphatidylcholine

A: Analyses of polysaccharides precipitated with 1.8 volumes of ethanol

Ethanol precipitation (V)

1.8

1.8

1.8

 1st peak

  Mw (kDa)

1710

Mw distributiona: 31.7% > 2000 kDa

1709

Mw distributiona: 32.1% > 2000 kDa

1983

Mw distributiona: 42.5% > 2000 kDa

  Mw/Mn

1.38

 

1.41

 

1.31

 

  IV (dL/g)

24.5

 

24.4

 

27.0

 

  Representativeness (%)

51.3

 

55.6

 

58.6

 

 2nd peak

  Mw (KDa)

720

 

241

 

511

 

  Mw/Mn

1.67

 

1.05

 

1.02

 

  IV (dL/g)

4.6

 

4.8

 

8.3

 

  Representativeness (%)

5.1

 

5.4

 

3.2

 

 3rd peak

  Mw (kDa)

53

Mw distributionb: 10 kDa < 48.7% < 50 kDa

51.3% > 50 kDa

41.8

Mw distributionb: 10 kDa < 48.3% < 50 kDa

51.7% > 50 kDa

70

Mw distributionb: 10 kDa < 66.0% < 50 kDa

34.0% > 50 kDa

  Mw/Mn

1.55

 

1.24

 

1.22

 

  IV (dL/g)

1.0

 

1.0

 

1.0

 

  Representativeness (%)

28.9

 

24.9

 

21.3

 

B: Analyses of polysaccharides precipitated with 4 volumes of ethanol

Ethanol precipitation (V)

4

4

4

 1st peak

  Mw (KDa)

27.9

27.5

27.6

  Mw/Mn

1.07

1.06

1.05

  IV (dL/g)

0.1

0.2

0.1

  Representativeness (%)

22.4

24.3

14.8

 2nd peak

  Mw

19.8

19.2

19.3

  Mw/Mn

1.02

1.01

1.02

  IV (dL/g)

0.1

0.1

0.1

  Representativeness (%)

77.5

75.5

85.1

  1. Mw/Mn polymer polydispersity, IV intrinsic viscosity
  2. aMolecular weight distribution of subpopulations found in the 1st peak indicating the percentage of molecules with a Mw > 2000 kDa
  3. bMolecular weight distribution of subpopulations found in the 3rd peak