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Table 2 SEC-TDA analyses of polysaccharides produced by the recombinant strain of S. equi subsp. zooepidemicus-pNZ8148kfoAkfoC during growth in shakeflasks on semidefined medium

From: Engineering S. equi subsp. zooepidemicus towards concurrent production of hyaluronic acid and chondroitin biopolymers of biomedical interest

  S. equi subsp. zooepidemicus-pNZ8148kfoAkfoC S. equi subsp. zooepidemicus-pNZ8148kfoAkfoC + 0.5 mM GalNAc S. equi subsp. zooepidemicus-pNZ8148kfoAkfoC + 2 mM phosphatidylcholine
A: Analyses of polysaccharides precipitated with 1.8 volumes of ethanol
Ethanol precipitation (V) 1.8 1.8 1.8
 1st peak
  Mw (kDa) 1710 Mw distributiona: 31.7% > 2000 kDa 1709 Mw distributiona: 32.1% > 2000 kDa 1983 Mw distributiona: 42.5% > 2000 kDa
  Mw/Mn 1.38   1.41   1.31  
  IV (dL/g) 24.5   24.4   27.0  
  Representativeness (%) 51.3   55.6   58.6  
 2nd peak
  Mw (KDa) 720   241   511  
  Mw/Mn 1.67   1.05   1.02  
  IV (dL/g) 4.6   4.8   8.3  
  Representativeness (%) 5.1   5.4   3.2  
 3rd peak
  Mw (kDa) 53 Mw distributionb: 10 kDa < 48.7% < 50 kDa
51.3% > 50 kDa
41.8 Mw distributionb: 10 kDa < 48.3% < 50 kDa
51.7% > 50 kDa
70 Mw distributionb: 10 kDa < 66.0% < 50 kDa
34.0% > 50 kDa
  Mw/Mn 1.55   1.24   1.22  
  IV (dL/g) 1.0   1.0   1.0  
  Representativeness (%) 28.9   24.9   21.3  
B: Analyses of polysaccharides precipitated with 4 volumes of ethanol
Ethanol precipitation (V) 4 4 4
 1st peak
  Mw (KDa) 27.9 27.5 27.6
  Mw/Mn 1.07 1.06 1.05
  IV (dL/g) 0.1 0.2 0.1
  Representativeness (%) 22.4 24.3 14.8
 2nd peak
  Mw 19.8 19.2 19.3
  Mw/Mn 1.02 1.01 1.02
  IV (dL/g) 0.1 0.1 0.1
  Representativeness (%) 77.5 75.5 85.1
  1. Mw/Mn polymer polydispersity, IV intrinsic viscosity
  2. aMolecular weight distribution of subpopulations found in the 1st peak indicating the percentage of molecules with a Mw > 2000 kDa
  3. bMolecular weight distribution of subpopulations found in the 3rd peak