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Table 1 S. equi subsp. zooepidemicus-pNZ8148kfoAkfoC grown in shakeflasks on semidefined medium

From: Engineering S. equi subsp. zooepidemicus towards concurrent production of hyaluronic acid and chondroitin biopolymers of biomedical interest

Condition OD600 Chondroitin extracellular (mg/L) Chondroitin intracellular (mg/L) YBC/Suc (mg/g) HA extracellular (mg/L) HA intracellular (mg/L) YHA/Suc (mg/g) YLA/Suc (g/g)
Nisin 20 ng/mL 8.2 ± 0.7 75 ± 8 13 ± 1
15%a
9.8 ± 0.3 412 ± 25 2.7 ± 0.2
0.6%a
47 ± 7 1.2 ± 0.1
Nisin 20 ng/mL, 0.5 mM GalNAc 8.7 ± 0.7 68 ± 6 24 ± 2
26%a
17.7 ± 1.5 366 ± 31 4.1 ± 0.4
1.1%a
71 ± 10 2.1 ± 0.2
Nisin 20 ng/mL, 2 mM phosphatidylcholine nd 44 ± 4 16 ± 2
27%a
5.8 ± 0.85 428 ± 38 2.7 ± 0.3
0.6%a
42 ± 6 1.2 ± 0.1
  1. Production of HA and chondroitin were analysed in the presence of inducer (nisin) alone or in combination with either GalNAc or phosphatidylcholine. Experiments lasted 18 h. Biomass and polymer concentrations indicated in the table were determined after 18 h of growth
  2. nd not detectable
  3. aPercentage of intracellular polymer on the total produced of each type (HA or chondroitin)