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Table 4 Summary of the rhamnolipids extracted from cultures of P. aeruginosa ST5, as detected with high resolution mass spectrometry (<10 ppm)

From: Characterisation and antimicrobial activity of biosurfactant extracts produced by Bacillus amyloliquefaciens and Pseudomonas aeruginosa isolated from a wastewater treatment plant

Rhamnolipid group (Abbr)

UPLC Rt (min)a

Proposed structures of rhamnolipids

Mono-isotopic Exp/Theor M r

Protonated specie Exp/Theor m/z

Sodiated specie Exp/Theor m/z

Sodiated dimeric specie Exp/Theor m/z

mRL1

7.23

Rha–C8–C10

Rha–C10–C8

476.3047

476.2985

477.3089

477.3063

499.2896

499.2883

975.5889

975.5868

dRL1

6.32

6.45

Rha–Rha–C8–C10

Rha–Rha–C10–C8

622.3576

622.3564

623.3654

623.3642

645.3471

645.3462

1267.7074

1267.7026

mRL2

8.77

8.84

Rha–C10–C10

504.3305

504.3298

505.3383

505.3376

527.3201

527.3196

1031.6501

1031.6494

dRL2

7.84

7.97

Rha–Rha–C10–C10

650.3894

650.3877

651.3972

651.3955

673.3772

673.3775

1323.7701

1323.7652

mRL3

10.32

Rha–C12–C10

Rha–C10–C12

532.3640

532.3611

533.3700

533.3689

555.3546

555.3509

1087.7201

1087.7120

dRL3

9.40

9.46

Rha–Rha–C12–C10

Rha–Rha–C10–C12

678.4177

678.4190

679.4285

679.4268

701.4114

701.4088

1379.8352

1379.8278

  1. Their proposed chemical structures, theoretical (Theor) and experimental (Exp) M r and monoisotopic m/z values, as well as observed UPLC retention times for representative examples are provided
  2. a UPLC retention time of main peaks corresponding to the group’s m/z value