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Table 4 Summary of the rhamnolipids extracted from cultures of P. aeruginosa ST5, as detected with high resolution mass spectrometry (<10 ppm)

From: Characterisation and antimicrobial activity of biosurfactant extracts produced by Bacillus amyloliquefaciens and Pseudomonas aeruginosa isolated from a wastewater treatment plant

Rhamnolipid group (Abbr) UPLC Rt (min)a Proposed structures of rhamnolipids Mono-isotopic Exp/Theor M r Protonated specie Exp/Theor m/z Sodiated specie Exp/Theor m/z Sodiated dimeric specie Exp/Theor m/z
mRL1 7.23 Rha–C8–C10
Rha–C10–C8
476.3047
476.2985
477.3089
477.3063
499.2896
499.2883
975.5889
975.5868
dRL1 6.32
6.45
Rha–Rha–C8–C10
Rha–Rha–C10–C8
622.3576
622.3564
623.3654
623.3642
645.3471
645.3462
1267.7074
1267.7026
mRL2 8.77
8.84
Rha–C10–C10 504.3305
504.3298
505.3383
505.3376
527.3201
527.3196
1031.6501
1031.6494
dRL2 7.84
7.97
Rha–Rha–C10–C10 650.3894
650.3877
651.3972
651.3955
673.3772
673.3775
1323.7701
1323.7652
mRL3 10.32 Rha–C12–C10
Rha–C10–C12
532.3640
532.3611
533.3700
533.3689
555.3546
555.3509
1087.7201
1087.7120
dRL3 9.40
9.46
Rha–Rha–C12–C10
Rha–Rha–C10–C12
678.4177
678.4190
679.4285
679.4268
701.4114
701.4088
1379.8352
1379.8278
  1. Their proposed chemical structures, theoretical (Theor) and experimental (Exp) M r and monoisotopic m/z values, as well as observed UPLC retention times for representative examples are provided
  2. a UPLC retention time of main peaks corresponding to the group’s m/z value