Table 1 Purification process of rBGLa
Purification steps | Total activity (U, μmol/min) | Total protein (mg) | Specific activity (U/mg) | Purification (fold) | Recovery (%) |
|---|---|---|---|---|---|
Crude cell lysate (soluble) | 4708 | 122.3 | 38.5 | 1 | 100 |
Affinity chromatography | 1578 | 11.1 | 142.0 | 3.7 | 33.5 |