Fig. 5

HPLC analysis for the bioconversion of Gardeniae Fructus hot-water extract using rBGLa. The enzymatic reaction of Gardeniae Fructus hot-water extract was performed in the reaction buffer at pH 6.0 and 45 °C. a Gardeniae Fructus extract (1.6 mg/mL) was reacted with free rBGLa (0.5 μg/mL), and the changes in geniposide and genipin concentration were monitored. b The transition of geniposide by immobilized rBGLa was monitored after 4-h reaction at 240 nm by HPLC, and was compared using standard geniposide and genipin