Table 1 Real-time PCR primers used in the relative quantification of ruminal microorganisms
Species | Sequence (5′–3′) | Amplicon size (bp) | Reference |
|---|---|---|---|
F. succinogenes | F: GGTATGGGATGAGCTTGC R: GCCTGCCCCTGAACTATC | 445 | Tajima et al. (2001) |
R. albus | F: CCCTAAAAGCAGTCTTAGTTCG R: CCTCCTTGCGGTTAGAACA | 175 | Wang et al. (1997) |
R. flavefaciens | F: TCTGGAAACGGATGGTA R: CCTTTAAGACAGGAGTTTACA | 295 | Koike and Kobaiashi (2001) |
S. bovis | F: CTAATACCGCATAACAGCAT R: AGAAACTTCCTATCTCTAGG | 127 | Stevenson and Weimer (2007) |
R. amylophilus | F: CAACCAGTCGCATTCAGA R: CACTACTCATGGCAACAT | 642 | Tajima et al. (2001) |
M. elsdenii | F: GACCGAAACTGCGATGCTAGA R: CGCCTCAGCGTCAGTTGTC | 129 | Ouwerkerk et al. (2002) |
Eubacteria | F: CCTACGGGAGGCAGCAG R: ATTACCGCGGCTGCTGG | 193 | Muyzer et al. (1993) |