Fig. 2

Effects of pH (a, c) and temperature (b, d) on BthChi74 activity. a The activity was measured at 50 °C in various buffers at different pH values as follows: 50 mM acetate buffer (pH 3.5–6.0, diamonds), 50 mM MES-NaOH buffer (pH 5.0–7.0, circles), or 50 mM Tris–HCl buffer (pH 7.0–8.0, triangles). b The activity was measured at various temperatures (30–70 °C). c BthChi74 was incubated at 4 °C for 24 h at various pH values as follows: 50 mM acetate buffer (pH 3.5–6.0, diamonds), 50 mM MES-NaOH buffer (pH 5.0–7.0, circles), 50 mM Tris–HCl buffer (pH 7.0–9.0, triangles), or 50 mM carbonate-NaOH buffer (pH 10–11, squares). The remaining activity was measured at 50 °C after diluting the pretreated enzymes in TS buffer. The average values with error bars are represented as the enzymatic activity. Experiments were performed in triplicate. d DSF analysis results for BthChi74 are shown. The experimental procedure is detailed in the “Materials and methods” section. Experiments were performed in triplicate, and the average values are shown