Fig. 6
a Confirmation of Lpp-OmpA-PE and Non-OmpA—PE fusion proteins displayed on the cell surface. Cells harboring Lpp-OmpA-PE and Non-OmpA–PE were incubated with anti-His-FITC antibody diluted (1:500) for 1 h at room temperature (top and center) Images show the localization of Non-OmpA-PE and Lpp-OmpA—PE fusion proteins respectively (down). No fluorescence signal was detected when the cells were not induced by IPTG. b SDS-PAGE analysis of the outer membrane proteins. Lane 1 is negative control. Lanes 2, 3 and 4 represent the outer membrane proteins isolated from E. coli BL21 (DE3) harboring pLpp-OmpA-PE, pNon-OmpA-PE, pNon-OmpA-PE (2×), respectively. Lane M represents molecular weight marker