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Fig. 5 | AMB Express

Fig. 5

From: Designing novel construction for cell surface display of protein E on Escherichia coli using non-classical pathway based on Lpp-OmpA

Fig. 5

Analysis of the expression of different chimeric proteins. a Total cell proteins were analyzed using SDS-PAGE.A: lanes 13 represent total cell proteins from E. coli BL21 (DE3) harboring pLpp-OmpA-PE, pPE22-160 and negative control, respectively. Lane M represents molecular weight markers. b The lane 1 is pNON-OmpA and lane 2 is pLpp-OmpA. Lane 3, 4 and 5 are pNon-OmpA, pPE22-160 and negative control, respectively. The last lane is protein marker c The presence of chimeric proteins in the total cell proteins from each clone was confirmed by Western blot using anti-His antibody. The bands corresponding to each chimeric proteins were detected in the lanes 28, as Non-OmpA (~16 kDa), Lpp-OmpA-PE (~30 kDa) from clones No. 1 and No. 4, protein E (~23 kDa) from clones No. 3 and No. 7 and Non-OmpA-PE (~30 kDa) from clones No. 3 and No. 5, respectively. Lane M represents molecular weight marker and lane 1 before induction

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