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Table 3 Composition of the LAMP reaction mix

From: Loop-mediated isothermal amplification of Neisseria gonorrhoeae porA pseudogene: a rapid and reliable method to detect gonorrhea

  Components Volume
Reaction mix 20 mM Tris–HCl (pH 8.8) 12.5 µL
10 mM KCl  
8 mM MgSO4  
10 mM (NH4)2SO4  
0.1% Tween-20  
1 mM Betaine  
1.6 mM dNTP  
Primer mix FIP (1.6 µM) and BIP (1.6 µM) 1 µL
F3 (0.2 µM) and B3 (0.2 µM)  
FLF (0.8 μM) and FLB (0.8 μM)  
Nuclease-free water   8 µL
Bst DNA polymerase   1 µL
SYTO-9   0.5 µL
DNA template   2 µL
Total volume   25 µL
  1. Reaction condition: 63 °C, 45–60 min