The synergistic effect of Selenium (selenite, –SeO 3 2− ) dose and irradiance intensity in Chlorella cultures

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Table 1 Changes of variables of the maximum PSII quantum yield, F_v/F_m, maximum electron transport rate rETR_max and the irradiance saturating photosynthesis E_k were estimated from rapid light-response curves (Additional file 1: Figure S1) monitored at midday on Day 1, 4 and 7 during a 7-day experiment in the presence and absence of Se

Culture treatment	Fluorescence variables (r.u.)	Day 1	Day 4	Day 7
Control (no Se)	F _v /F _m	0.77	0.75	0.80
	rETR _max	461	404	359
	E _k	758	714	531
	V _j	0.21	0.22	0.21
	V _i	0.43	0.43	0.43
Se treated culture	F _v /F _m	0.74	0.74	0.78
	rETR _max	436	380	332
	E _k	665	660	625
	V _j	0.24	0.26	0.23
	V _i	0.46	0.46	0.46

Changes of variables Vj and Vi were calculated from fast fluorescence induction kinetics (Fig. S2) monitored on Day 1, 4 and 7 during a 10-day experiment in the presence and absence of Se. Vj reflects a reduction of Q _A ⁻ (photochemical phase) while Vi is thought to correspond reflect various redox state of temporary maximum of Q _A ⁻ Q _B ²⁻