Fig. 4

Gene expression and intracellular erythritol accumulation under short-term osmotic stress with 20% glucose in Moniliella megachiliensis. a Levels of gene expression were analyzed by semi-quantitative PCR analysis; data provided are representative ultraviolet-illuminated bands following electrophoresis in ethidium bromide-stained agarose gels. b Intracellular erythritol content is presented as µg/mg dry cells (mean ± SD from three independent determinations). When OD₆₀₀ reached approximately 1.0, glucose was added to the cell culture to a final concentration of 20%, and the culture was incubated for 120 min at 30 °C. Gene expression was analyzed by semi-quantitative PCR using GAPDH as a standard. Intracellular erythritol content was chromatographically determined with an ICS-3000 system, and is represented as µg/mg dry cells