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Table 1 Physiological characterization related parameters of the PHB-producing strains TMB4443 and TMB4425 under aerobic and anaerobic conditions, respectively

From: Effect of nitrogen availability on the poly-3-d-hydroxybutyrate accumulation by engineered Saccharomyces cerevisiae

Strain C-source  Nitrogen supply Aeration Initial CDW (g/L) Yields PHB titer
(g/L)
PHB content
(% PHB/CDW)
YsX
(g/g C-source)
YsXylOH
(g/g C-source)
YsGlyOH
(g/g C-source)
YsAcet
(g/g C-source)
YsEtOH
(g/g C-source)
YsPHB
(mg/g C-source)
TMB4443 Glucose No Aerobic 3.8 ± 0.0 0.06 ± 0.00 0.00 ± 0.00 0.04 ± 0.01 0.01 ± 0.00 0.04 ± 0.01 1.0 ± 0.1 0.12 ± 0.00 1.8 ± 0.0
TMB4443 Glucose Yes Aerobic 0.01 ± 0.00 0.09 ± 0.01 0.00 ± 0.00 0.05 ± 0.01 0.03 ± 0.01 0.14 ± 0.07 2.12 ± 0.04 0.11 ± 0.00 2.4 ± 0.0
TMB4425 Xylose No Anaerobic 4.1 ± 0.2 0.06 ± 0.01 0.02 ± 0.00 0.09 ± 0.01 0.01 ± 0.00 0.01 ± 0.01 8.3 ± 0.8 0.43 ± 0.00 6.1 ± 0.1
TMB4425 Xylose Yes Anaerobic 0.02 ± 0.00 0.09 ± 0.00 0.04 ± 0.00 0.04 ± 0.00 0.01 ± 0.00 0.21 ± 0.03 13.8 ± 0.6 0.73 ± 0.00 16.4 ± 0.8
  1. Cell dry weight (CDW), volumetric yields and titers were calculated from a single time-point corresponding to the end of the experiment (varied from 141 to 172 h). Values reported in the table represent the mean ± SD of at least two independent biological replicates. Glucose or xylose was used as sole C-source
  2. YSX: biomass yield on C-source, YSXylOH: xylitol yield on C-source, YSGlyOH: glycerol yield on C-source, YSAcet: acetate yield on C-source, YSEtOH: ethanol yield on C-source and YSPHB: PHB yield on C-source. PHB titer: final PHB concentration. PHB content: percentage of PHB accumulated in the biomass formed